Analysis of diacylglycerol molecular species in cellular lipid extracts by normal-phase LC-electrospray mass spectrometry
TJ Leiker, RM Barkley, RC Murphy - International journal of mass …, 2011 - Elsevier
The quantitative determination of 48 molecular species of regioisomeric diacylglycerols has
been made in a single analysis of an extract of bone marrow derived macrophages. The
analytical procedure involves solvent extraction of neutral lipids, including diacylglycerols,
derivatization of free hydroxyl moieties as 2, 4-difluorophenyl urethane, and analysis by
normal phase liquid chromatography–tandem mass spectrometry. The derivatization step
not only prevents fatty acyl group migration, thus allowing determination of both 1, 2-and 1, 3 …
been made in a single analysis of an extract of bone marrow derived macrophages. The
analytical procedure involves solvent extraction of neutral lipids, including diacylglycerols,
derivatization of free hydroxyl moieties as 2, 4-difluorophenyl urethane, and analysis by
normal phase liquid chromatography–tandem mass spectrometry. The derivatization step
not only prevents fatty acyl group migration, thus allowing determination of both 1, 2-and 1, 3 …