Cell growth and the initiation of transformation by SV40.
GJ Todaro, H Green - … of the National Academy of Sciences, 1966 - National Acad Sciences
GJ Todaro, H Green
Proceedings of the National Academy of Sciences, 1966•National Acad SciencesMaterials and Methods.-Fibroblast line 3T3, a spontaneously established cell line of mouse
embryo origin, 3 was used between the 105th and 200th cell generation. Cells were
infectedwith SV40 strain 776, grown on Rhesus monkey kidney cultures4 and
kindlysupplied by Dr. John Easton (National Institutes of Health). The transformation assay
using SV40 and cell line 3T3 has been described in detail. 5'6 The transformed colonies
may be scored, after confluence is attained, against a uniform background of untransformed …
embryo origin, 3 was used between the 105th and 200th cell generation. Cells were
infectedwith SV40 strain 776, grown on Rhesus monkey kidney cultures4 and
kindlysupplied by Dr. John Easton (National Institutes of Health). The transformation assay
using SV40 and cell line 3T3 has been described in detail. 5'6 The transformed colonies
may be scored, after confluence is attained, against a uniform background of untransformed …
Materials and Methods.-Fibroblast line 3T3, a spontaneously established cell line of mouse embryo origin, 3 was used between the 105th and 200th cell generation. Cells were infectedwith SV40 strain 776, grown on Rhesus monkey kidney cultures4 and kindlysupplied by Dr. John Easton (National Institutes of Health). The transformation assay using SV40 and cell line 3T3 has been described in detail. 5'6 The transformed colonies may be scored, after confluence is attained, against a uniform background of untransformed cells. Results.-(1) Cell growth and the expression of the transformed state: The transformation of 3T3 by SV40 has usually been studied byinfecting a population of cells and inoculating them on the following day at high dilution into a number
National Acad Sciences