Related B cell clones that populate the CSF and CNS of patients with multiple sclerosis produce CSF immunoglobulin

B Obermeier, L Lovato, R Mentele, W Brück… - Journal of …, 2011 - Elsevier
We investigated the overlap shared between the immunoglobulin (Ig) proteome of the
cerebrospinal fluid (CSF) and the B cell Ig-transcriptome of CSF and the central nervous
system (CNS) tissue of three patients with multiple sclerosis. We determined the IgG-
proteomes of CSF by mass spectrometry, and compared them to the IgG-transcriptomes from
CSF and brain lesions, which were analyzed by cDNA cloning. Characteristic peptides that
were identified in the CSF-proteome could also be detected in the transcriptomes of both …