[HTML][HTML] In vivo effects of uncoupling protein-3 gene disruption on mitochondrial energy metabolism
GW Cline, AJ Vidal-Puig, S Dufour, KS Cadman… - Journal of Biological …, 2001 - ASBMB
To clarify the role of uncoupling protein-3 (UCP3) in skeletal muscle, we used NMR and
isotopic labeling experiments to evaluate the effect of UCP3 knockout (UCP3KO) in mice on
the regulation of energy metabolism in vivo. Whole body energy expenditure was
determined from the turnover of doubly labeled body water. Coupling of mitochondrial
oxidative phosphorylation in skeletal muscle was evaluated from measurements of rates of
ATP synthesis (using 31 P NMR magnetization transfer experiments) and tricarboxylic acid …
isotopic labeling experiments to evaluate the effect of UCP3 knockout (UCP3KO) in mice on
the regulation of energy metabolism in vivo. Whole body energy expenditure was
determined from the turnover of doubly labeled body water. Coupling of mitochondrial
oxidative phosphorylation in skeletal muscle was evaluated from measurements of rates of
ATP synthesis (using 31 P NMR magnetization transfer experiments) and tricarboxylic acid …