[HTML][HTML] Visualizing priming of virus-specific CD8+ T cells by infected dendritic cells in vivo

CC Norbury, D Malide, JS Gibbs, JR Bennink… - Nature …, 2002 - nature.com
CC Norbury, D Malide, JS Gibbs, JR Bennink, JW Yewdell
Nature immunology, 2002nature.com
The rational design of vaccines that elicit CD8+ T cell responses requires knowledge of the
identity of the antigen-presenting cell (APC), the location and time of presentation and the
nature of the antigen presented by the APC. Here we address these questions for an antigen
encoded by a recombinant vaccinia virus. We found that, following local infection, vaccinia
virus infected macrophages and dendritic cells in draining lymph nodes. However, only the
dendritic cells presented antigen to naļve CD8+ T cells, as determined by direct visualization …
Abstract
The rational design of vaccines that elicit CD8+ T cell responses requires knowledge of the identity of the antigen-presenting cell (APC), the location and time of presentation and the nature of the antigen presented by the APC. Here we address these questions for an antigen encoded by a recombinant vaccinia virus. We found that, following local infection, vaccinia virus infected macrophages and dendritic cells in draining lymph nodes. However, only the dendritic cells presented antigen to naļve CD8+ T cells, as determined by direct visualization of sectioned nodes by confocal microscopy. Presentation occurred as rapidly as 6 h after inoculation and quickly declined in parallel with the number of infected cells present in the nodes. These data provide direct evidence that virus-infected APCs prime naļve CD8+ T cells in vivo.
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