Triglyceride synthesis in mammalian tissues requires glycerol 3-phosphate as the source of
triglyceride glycerol. In this study the relative contribution of glyceroneogenesis and
glycolysis to triglyceride glycerol synthesis was quantified in vivo in adipose tissue, skeletal
muscle, and liver of the rat in response to a chow diet (controls), 48-h fast, and lipogenic
(high sucrose) diet. The rate of glyceroneogenesis was quantified using the tritium ([3 H 2]
O) labeling of body water, and the contribution of glucose, via glycolysis, was determined …