Tumor-stroma interaction of human pancreatic cancer: acquired resistance to anticancer drugs and proliferation regulation is dependent on extracellular matrix …

H Miyamoto, T Murakami, K Tsuchida, H Sugino… - Pancreas, 2004 - journals.lww.com
H Miyamoto, T Murakami, K Tsuchida, H Sugino, H Miyake, S Tashiro
Pancreas, 2004journals.lww.com
Aims To examine the role of ECM proteins in acquired resistance to anticancer drugs and
proliferation regulation in pancreatic cancers. Methodology and Results We used an in vitro
model of ECM-induced chemoresistance and cell proliferation of cancer cell lines (MIA
PaCa-2, PANC-1, and Capan-1) with 3 different malignancy grades and found that
resistance to cytotoxic drugs and proliferation regulation was dependent on ECM proteins.
Pancreatic cancer cell lines, especially MIA PaCa-2 cells, adhering to any of the ECM …
Aims To examine the role of ECM proteins in acquired resistance to anticancer drugs and proliferation regulation in pancreatic cancers.
Methodology and Results We used an in vitro model of ECM-induced chemoresistance and cell proliferation of cancer cell lines (MIA PaCa-2, PANC-1, and Capan-1) with 3 different malignancy grades and found that resistance to cytotoxic drugs and proliferation regulation was dependent on ECM proteins. Pancreatic cancer cell lines, especially MIA PaCa-2 cells, adhering to any of the ECM proteins showed decreased cytotoxicity of anticancer drugs, except for gemcitabine. PANC-1 and Capan-1 cells adhering to fibronectin, collagen I, and collagen IV proliferated more than the controls.
Conclusion ECM proteins have important roles in acquired resistance to anticancer drugs and cell proliferation regulation of pancreatic cancer cells. Therefore, the expression of ECM proteins in pancreatic cancer specimens could provide valuable information to aid anticancer drug cytotoxicity, and gemcitabine would be useful for treatment of patients with pancreatic cancer.
Lippincott Williams & Wilkins