Biologic and clinical importance of proinsulin

DC Robbins, HS Tager… - New England Journal of …, 1984 - Mass Medical Soc
DC Robbins, HS Tager, AH Rubenstein
New England Journal of Medicine, 1984Mass Medical Soc
IN 1953 Sanger and Thompson described the complete covalent structure of insulin, a
hormone containing two separate peptide chains and three disulfide bonds. 1, 2 Although
proposed models for the biosynthesis of such a complex molecule were debated for many
years, it was not until 1967 that the mechanism for insulin biosynthesis was finally
elucidated: using an in vitro system containing isolated pancreatic islets or human
insulinoma tissue and Radio-labeled amino acids, Steiner and his colleagues showed that …
IN 1953 Sanger and Thompson described the complete covalent structure of insulin, a hormone containing two separate peptide chains and three disulfide bonds.1,2 Although proposed models for the biosynthesis of such a complex molecule were debated for many years, it was not until 1967 that the mechanism for insulin biosynthesis was finally elucidated: using an in vitro system containing isolated pancreatic islets or human insulinoma tissue and Radio-labeled amino acids, Steiner and his colleagues showed that the formation of insulin was preceded by the biosynthesis of a single-chain insulin-containing peptide about 1 1/2 times the size of insulin itself.3,4 Isolation . . .
The New England Journal Of Medicine