[HTML][HTML] Conditional mutagenesis of the murine serum response factor gene blocks cardiogenesis and the transcription of downstream gene targets
Serum response factor (SRF) homozygous-null embryos from our backcross of SRF LacZ/+"
knock-in" mice failed to gastrulate and form mesoderm, similar to the findings of an earlier
study (Arsenian, S., Weinhold, B., Oelgeschlager, M., Ruther, U., and Nordheim, A.(1998)
EMBO J. 17, 6289–6299). Our use of embryonic stem cells provided a model system that
could be used to investigate the specification of multiple embryonic lineages, including
cardiac myocytes. We observed the absence of myogenic α-actins, SM22α, and myocardin …
knock-in" mice failed to gastrulate and form mesoderm, similar to the findings of an earlier
study (Arsenian, S., Weinhold, B., Oelgeschlager, M., Ruther, U., and Nordheim, A.(1998)
EMBO J. 17, 6289–6299). Our use of embryonic stem cells provided a model system that
could be used to investigate the specification of multiple embryonic lineages, including
cardiac myocytes. We observed the absence of myogenic α-actins, SM22α, and myocardin …