[HTML][HTML] Defining the functional boundaries of the Gata2 locus by rescue with a linked bacterial artificial chromosome transgene
W Brandt, M Khandekar, N Suzuki, M Yamamoto… - Journal of biological …, 2008 - ASBMB
Transcription factor GATA-2 is vital for both hematopoietic progenitor cell function and
urogenital patterning. Transgenic mapping studies have shown that the hematopoietic and
urogenital enhancers are located hundreds of kbp 5′ and 3′ to the Gata2 structural gene,
and both are vital for embryonic development. Because the size of mammalian genes,
including all of their associated regulatory elements, can exceed a megabase, transgenic
complementation in mice has, in specific instances, proven to be a formidable hurdle. After …
urogenital patterning. Transgenic mapping studies have shown that the hematopoietic and
urogenital enhancers are located hundreds of kbp 5′ and 3′ to the Gata2 structural gene,
and both are vital for embryonic development. Because the size of mammalian genes,
including all of their associated regulatory elements, can exceed a megabase, transgenic
complementation in mice has, in specific instances, proven to be a formidable hurdle. After …