Genetic characterization of human Dsg3-specific B cells isolated by flow cytometry from the peripheral blood of patients with pemphigus vulgaris
BACKGROUND: Pemphigus vulgaris (PV) is an autoimmune bullous disease caused by anti-
desmoglein 3 (Dsg3) IgG autoantibodies; however, the Dsg3-specific B cells that produce
anti-Dsg3 IgG are not well characterized. OBJECTIVES: Our aims were to develop a flow
cytometric method for the isolation of Dsg3-specific B cells from the peripheral blood of
patients with active PV and to identify the variable regions within their heavy-and light-chain
immunoglobulin genes. METHODS: Dsg3-specific B cells were isolated as CD3− IgD− PI …
desmoglein 3 (Dsg3) IgG autoantibodies; however, the Dsg3-specific B cells that produce
anti-Dsg3 IgG are not well characterized. OBJECTIVES: Our aims were to develop a flow
cytometric method for the isolation of Dsg3-specific B cells from the peripheral blood of
patients with active PV and to identify the variable regions within their heavy-and light-chain
immunoglobulin genes. METHODS: Dsg3-specific B cells were isolated as CD3− IgD− PI …