[HTML][HTML] Assembly pathway of desmoglein 3 to desmosomes and its perturbation by pemphigus vulgaris-IgG in cultured keratinocytes, as revealed by time-lapsed …
M Sato, Y Aoyama, Y Kitajima - Laboratory investigation, 2000 - Elsevier
To determine the assembly pathway of desmoglein 3 (Dsg3) into desmosomes and the
subsequent effects of pemphigus vulgaris immunoglobulin G (PV-IgG) on such, we
employed a time-lapsed labeling for FITC/Rhodamine (Rod) double-stained
immunofluorescence and 5-nm/10-nm gold double-stained immunoelectron microscopy by
using PV-IgG, which was confirmed to react specifically Dsg3. Cells from a human
squamous cell carcinoma cell line (DJM-1) were first treated briefly with PV-IgG (3 min), then …
subsequent effects of pemphigus vulgaris immunoglobulin G (PV-IgG) on such, we
employed a time-lapsed labeling for FITC/Rhodamine (Rod) double-stained
immunofluorescence and 5-nm/10-nm gold double-stained immunoelectron microscopy by
using PV-IgG, which was confirmed to react specifically Dsg3. Cells from a human
squamous cell carcinoma cell line (DJM-1) were first treated briefly with PV-IgG (3 min), then …