[PDF][PDF] BCL-2/JH rearrangements in circulating B cells of healthy blood donors and patients with nonmalignant diseases.
G Dölken, G Illerhaus, C Hirt… - Journal of Clinical …, 1996 - researchgate.net
G Dölken, G Illerhaus, C Hirt, R Mertelsmann
Journal of Clinical Oncology, 1996•researchgate.netPurpose: To answer the question whether t (14; 18)-positive cells can be detected by
polymerase chain reaction (PCR) in the peripheral blood of healthy blood donors and
patients with nonmalignant diseases. Patients and Methods: Peripheral-blood mononu-clear
cells (PBMNC) from healthy donors (n= 36) and patients with nonmalignant diseases (n= 21)
were examined by two-step PCR for the detection of t (14; 18)-positive cells with a
breakpoint within the major breakpoint region (MBR). Approximate numbers of t (14; 18) …
polymerase chain reaction (PCR) in the peripheral blood of healthy blood donors and
patients with nonmalignant diseases. Patients and Methods: Peripheral-blood mononu-clear
cells (PBMNC) from healthy donors (n= 36) and patients with nonmalignant diseases (n= 21)
were examined by two-step PCR for the detection of t (14; 18)-positive cells with a
breakpoint within the major breakpoint region (MBR). Approximate numbers of t (14; 18) …
Purpose: To answer the question whether t (14; 18)-positive cells can be detected by polymerase chain reaction (PCR) in the peripheral blood of healthy blood donors and patients with nonmalignant diseases.
Patients and Methods: Peripheral-blood mononu-clear cells (PBMNC) from healthy donors (n= 36) and patients with nonmalignant diseases (n= 21) were examined by two-step PCR for the detection of t (14; 18)-positive cells with a breakpoint within the major breakpoint region (MBR). Approximate numbers of t (14; 18)-positive cells were determined using limiting dilution assays, as well as the stochastic multiple-tube approach.
Results: We were able to detect t (14; 1 8)-positive cells in PBMNC of approximately 50% of healthy donors and patients with nonmalignant diseases if DNA amounts up to 10 jig were tested. Compared with 17 t (14; 18)-positive patients being in complete remission after radiother-
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