[HTML][HTML] In vivo measurement of microtubule dynamics using stable isotope labeling with heavy water: effect of taxanes
P Fanara, S Turner, R Busch, S Killion, M Awada… - Journal of Biological …, 2004 - ASBMB
Microtubules are dynamic polymers with central roles in the mitotic checkpoint, mitotic
spindle assembly, and chromosome segregation. Agents that block mitotic progression and
cell proliferation by interfering with microtubule dynamics (microtubule-targeted tubulin-
polymerizing agents (MTPAs)) are powerful antitumor agents. Effects of MTPAs (eg
paclitaxel) on microtubule dynamics have not yet been directly demonstrated in intact
animals, however. Here we describe a method that measures microtubule dynamics as an …
spindle assembly, and chromosome segregation. Agents that block mitotic progression and
cell proliferation by interfering with microtubule dynamics (microtubule-targeted tubulin-
polymerizing agents (MTPAs)) are powerful antitumor agents. Effects of MTPAs (eg
paclitaxel) on microtubule dynamics have not yet been directly demonstrated in intact
animals, however. Here we describe a method that measures microtubule dynamics as an …