Regulation of the Cl−/HCO3− exchanger AE2 in rat thick ascending limb of Henle's loop in response to changes in acid-base and sodium balance
F Quentin, D Eladari, S Frische… - Journal of the …, 2004 - journals.lww.com
Abstract The Cl−/HCO 3− exchanger AE2 is believed to be involved in transcellular
bicarbonate reabsorption that occurs in the thick ascending limb of Henle's loop (TAL). The
purpose of this study was to test whether chronic changes in acid-base status and sodium
intake regulate AE2 polypeptide abundance in the TAL of the rat. Rats were subjected to 6 d
of loading with NaCl, NH 4 Cl, NaHCO 3, KCl, or KHCO 3. AE2 protein abundance was
estimated by semiquantitative immunoblotting in renal membrane fractions isolated from the …
bicarbonate reabsorption that occurs in the thick ascending limb of Henle's loop (TAL). The
purpose of this study was to test whether chronic changes in acid-base status and sodium
intake regulate AE2 polypeptide abundance in the TAL of the rat. Rats were subjected to 6 d
of loading with NaCl, NH 4 Cl, NaHCO 3, KCl, or KHCO 3. AE2 protein abundance was
estimated by semiquantitative immunoblotting in renal membrane fractions isolated from the …