Cidec is a lipid droplet-associated protein, which inhibits lipolysis, leading to the accumulation of triglycerides in adipocytes. However, the transcriptional regulation of Cidec in adipocyte remains unknown. In the present study we investigated that the mouse Cidec transcript is regulated by PPARγ2. After the differentiation of adipocyte, the expression pattern of Cidec was similar to that of PPARγ2. In the presence of a PPARγ agonist, the level of Cidec mRNA was highly increased. In addition, putative PPRE sites were identified in the Cidec promoter. By chromatin immunoprecipitation assay and reporter assay, we observed the binding of PPARγ2 to the promoter of Cidec. Gel shift assay and the mutagenesis study were showed that the −219/−207 region of the Cidec promoter could function as a PPRE of the Cidec promoter. These results suggest that PPARγ2 is required for the transcriptional activity of Cidec during adipogenesis, which could be contributed to understand the molecular mechanism of lipid droplet formation in adipocytes.