[HTML][HTML] HCLK2 is required for activity of the DNA damage response kinase ATR
JMR Danielsen, DH Larsen, R Freire, J Falck… - Journal of biological …, 2009 - ASBMB
ATR is a protein kinase that orchestrates the cellular response to replication problems and
DNA damage. HCLK2 has previously been reported to stabilize ATR and Chk1. Here we
provide evidence that human HCLK2 acts at an early step in the ATR signaling pathway and
contributes to full-scale activation of ATR kinase activity. We show that HCLK2 forms a
complex with ATR-ATRIP and the ATR activator TopBP1. We demonstrate that HCLK2-
induced ATR kinase activity toward substrates requires TopBP1 and vice versa and provides …
DNA damage. HCLK2 has previously been reported to stabilize ATR and Chk1. Here we
provide evidence that human HCLK2 acts at an early step in the ATR signaling pathway and
contributes to full-scale activation of ATR kinase activity. We show that HCLK2 forms a
complex with ATR-ATRIP and the ATR activator TopBP1. We demonstrate that HCLK2-
induced ATR kinase activity toward substrates requires TopBP1 and vice versa and provides …