[HTML][HTML] Lithium stabilizes the CCAAT/enhancer-binding protein α (C/EBPα) through a glycogen synthase kinase 3 (GSK3)-independent pathway involving direct …
M Shim, RC Smart - Journal of Biological Chemistry, 2003 - ASBMB
CCAAT/enhancer-binding protein α (C/EBPα), a basic leucine zipper transcription factor, is
involved in mitotic growth arrest and differentiation. Given that numerous proteins involved in
cell cycle regulation are degraded via the ubiquitin-proteasome system, we examined
whether the C/EBPα protein is degraded via a proteasomal mechanism. In cycloheximide-
treated BALB/MK2 keratinocytes we found that C/EBPα is a short-lived protein with a half-life
of∼ 1 h. Treatment with proteasome inhibitors, MG-132 or lactacystin, blocked the …
involved in mitotic growth arrest and differentiation. Given that numerous proteins involved in
cell cycle regulation are degraded via the ubiquitin-proteasome system, we examined
whether the C/EBPα protein is degraded via a proteasomal mechanism. In cycloheximide-
treated BALB/MK2 keratinocytes we found that C/EBPα is a short-lived protein with a half-life
of∼ 1 h. Treatment with proteasome inhibitors, MG-132 or lactacystin, blocked the …