[HTML][HTML] Differential metabolic effects of adenovirus-mediated glucokinase and hexokinase I overexpression in rat primary hepatocytes
RM O'Doherty, DL Lehman, J Seoane… - Journal of Biological …, 1996 - ASBMB
The first step of glucose metabolism is the phosphorylation of glucose, catalyzed by the
hexokinase family of enzymes. To address the metabolic impact of increasing glucose
phosphorylation capacity in liver, rat primary hepatocytes were treated with recombinant
adenoviruses containing the cDNAs encoding either rat liver glucokinase (AdCMV-GKL) or
rat hexokinase I (AdCMV-HKI). Maximal glucose phosphorylation in AdCMV-GKL-and
AdCMV-HKI-treated hepatocytes was increased 7.1±1.2-and 6.3±0.8-fold, respectively, over …
hexokinase family of enzymes. To address the metabolic impact of increasing glucose
phosphorylation capacity in liver, rat primary hepatocytes were treated with recombinant
adenoviruses containing the cDNAs encoding either rat liver glucokinase (AdCMV-GKL) or
rat hexokinase I (AdCMV-HKI). Maximal glucose phosphorylation in AdCMV-GKL-and
AdCMV-HKI-treated hepatocytes was increased 7.1±1.2-and 6.3±0.8-fold, respectively, over …