New immunoaffinity-LC-MS/MS methodology reveals that Aag null mice are deficient in their ability to clear 1, N6-etheno-deoxyadenosine DNA lesions from lung and …
The mouse alkyladenine DNA glycosylase (Aag) initiates base excision repair with a broad
substrate range that includes the highly mutagenic exocyclic etheno DNA base adduct 1, N6-
ethenodeoxyadenosine (εdA). Previous attempts to determine the in vivo role of Aag in εdA
repair were complicated by technological difficulties in measuring low levels of εdA in
genomic DNA. Here we describe the development of a new method for εdA detection in
genomic DNA that couples an immunoaffinity purification with LC-MS/MS analysis and that …
substrate range that includes the highly mutagenic exocyclic etheno DNA base adduct 1, N6-
ethenodeoxyadenosine (εdA). Previous attempts to determine the in vivo role of Aag in εdA
repair were complicated by technological difficulties in measuring low levels of εdA in
genomic DNA. Here we describe the development of a new method for εdA detection in
genomic DNA that couples an immunoaffinity purification with LC-MS/MS analysis and that …