Asc1p, a WD40-domain containing adaptor protein, is required for the interaction of the RNA-binding protein Scp160p with polysomes

S Baum, M Bittins, S Frey, M Seedorf - Biochemical Journal, 2004 - portlandpress.com
S Baum, M Bittins, S Frey, M Seedorf
Biochemical Journal, 2004portlandpress.com
Scp160p interacts in an mRNA-dependent manner with translating ribosomes via multiple
RNA-binding heterogeneous nuclear ribonucleoprotein K-homology (KH) domains. In the
present study, we show by protein–protein cross-linking that Scp160p is in close proximity to
translation elongation factor 1A and the WD40 (Trp-Asp 40)-repeat containing protein Asc1p
at ribosomes. Analysis of a truncation mutant revealed that the C-terminus of Scp160p is
essential for ribosome binding and that Cys1067 at the C-terminus of Scp160p is required to …
Scp160p interacts in an mRNA-dependent manner with translating ribosomes via multiple RNA-binding heterogeneous nuclear ribonucleoprotein K-homology (KH) domains. In the present study, we show by protein–protein cross-linking that Scp160p is in close proximity to translation elongation factor 1A and the WD40 (Trp-Asp 40)-repeat containing protein Asc1p at ribosomes. Analysis of a truncation mutant revealed that the C-terminus of Scp160p is essential for ribosome binding and that Cys1067 at the C-terminus of Scp160p is required to obtain these cross-links. The interaction of Scp160p with ribosomes depends on Asc1p. In fast-growing yeast cells, nearly all Asc1p is tightly bound to ribosomes, but it can also be present in a ribosome-free form depending on growth conditions. The functional homologue of Asc1p, mammalian RACK1 (receptor of activated C kinase), was previously characterized as an adaptor protein bridging activated signalling molecules with their substrates. Our results suggest that Scp160p connects specific mRNAs, ribosomes and a translation factor with an adaptor for signalling molecules. These interactions might regulate the translation activity of ribosomes programmed with specific mRNAs.
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