Targeting neural circuitry in zebrafish using GAL4 enhancer trapping
EK Scott, L Mason, AB Arrenberg, L Ziv, NJ Gosse… - Nature …, 2007 - nature.com
Nature methods, 2007•nature.com
We present a pilot enhancer trap screen using GAL4 to drive expression of upstream
activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous
enhancers in zebrafish. The patterns presented include expression in small subsets of
neurons throughout the larval brain, which in some cases persist into adult. Through
targeted photoconversion of UAS-driven Kaede and variegated expression of UAS-driven
GFP in single cells, we begin to characterize the cellular components of labeled circuits.
activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous
enhancers in zebrafish. The patterns presented include expression in small subsets of
neurons throughout the larval brain, which in some cases persist into adult. Through
targeted photoconversion of UAS-driven Kaede and variegated expression of UAS-driven
GFP in single cells, we begin to characterize the cellular components of labeled circuits.
Abstract
We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult. Through targeted photoconversion of UAS-driven Kaede and variegated expression of UAS-driven GFP in single cells, we begin to characterize the cellular components of labeled circuits.
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