[HTML][HTML] Pax7 is required for the specification of myogenic satellite cells

P Seale, LA Sabourin, A Girgis-Gabardo, A Mansouri… - Cell, 2000 - cell.com
P Seale, LA Sabourin, A Girgis-Gabardo, A Mansouri, P Gruss, MA Rudnicki
Cell, 2000cell.com
The paired box transcription factor Pax7 was isolated by representational difference analysis
as a gene specifically expressed in cultured satellite cell-derived myoblasts. In situ
hybridization revealed that Pax7 was also expressed in satellite cells residing in adult
muscle. Cell culture and electron microscopic analysis revealed a complete absence of
satellite cells in Pax7−/− skeletal muscle. Surprisingly, fluorescence-activated cell sorting
analysis indicated that the proportion of muscle-derived stem cells was unaffected …
Abstract
The paired box transcription factor Pax7 was isolated by representational difference analysis as a gene specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization revealed that Pax7 was also expressed in satellite cells residing in adult muscle. Cell culture and electron microscopic analysis revealed a complete absence of satellite cells in Pax7−/− skeletal muscle. Surprisingly, fluorescence-activated cell sorting analysis indicated that the proportion of muscle-derived stem cells was unaffected. Importantly, stem cells from Pax7−/− muscle displayed almost a 10-fold increase in their ability to form hematopoietic colonies. These results demonstrate that satellite cells and muscle-derived stem cells represent distinct cell populations. Together these studies suggest that induction of Pax7 in muscle-derived stem cells induces satellite cell specification by restricting alternate developmental programs.
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