Assessment of chemokine receptor function on monocytes in whole blood: In vitro and ex vivo evaluations of a CCR2 antagonist
T Wisniewski, E Bayne, J Flanagan, Q Shao… - Journal of …, 2010 - Elsevier
Inhibition of monocyte and macrophage function by targeting chemokine receptors
represents an attractive strategy for therapeutic intervention in inflammatory diseases. We
describe an assay to assess chemokine receptor function on whole blood monocytes by
measuring chemokine stimulated change in cell shape as measured by flow cytometry. The
relative potential of the chemokine receptors CCR1, CCR2, CCR5, CX3CR1, and CXCR4 to
activate monocytes in whole blood was evaluated and compared. Analysis of MCP-1 …
represents an attractive strategy for therapeutic intervention in inflammatory diseases. We
describe an assay to assess chemokine receptor function on whole blood monocytes by
measuring chemokine stimulated change in cell shape as measured by flow cytometry. The
relative potential of the chemokine receptors CCR1, CCR2, CCR5, CX3CR1, and CXCR4 to
activate monocytes in whole blood was evaluated and compared. Analysis of MCP-1 …