[HTML][HTML] Phosphorylation of Mcm4 at specific sites by cyclin-dependent kinase leads to loss of Mcm4, 6, 7 helicase activity
Y Ishimi, Y Komamura-Kohno - Journal of Biological Chemistry, 2001 - ASBMB
Mcm proteins that play an essential role in eukaryotic DNA replication are phosphorylated in
vivo, and cyclin-dependent protein kinase is at least in part responsible for the
phosphorylation of Mcm4. Our group reported that the DNA helicase activity of Mcm4, 6, 7
complex, which may be involved in initiation of DNA replication, is inhibited following
phosphorylation by Cdk2/cyclin A in vitro. Here, we further examined the interplay between
mouse Mcm4, 6, 7 complex and cyclin-dependent kinases and determined the sites required …
vivo, and cyclin-dependent protein kinase is at least in part responsible for the
phosphorylation of Mcm4. Our group reported that the DNA helicase activity of Mcm4, 6, 7
complex, which may be involved in initiation of DNA replication, is inhibited following
phosphorylation by Cdk2/cyclin A in vitro. Here, we further examined the interplay between
mouse Mcm4, 6, 7 complex and cyclin-dependent kinases and determined the sites required …