Multiplexed labeling of samples with cell tracking dyes facilitates rapid and accurate internally controlled calcium flux measurement by flow cytometry
G Fu, NRJ Gascoigne - Journal of immunological methods, 2009 - Elsevier
Calcium flux measurement is a crucial assay in lymphocyte activation. However, with the
currently well established flow cytometric methods, it is a tedious procedure that is difficult to
control to avoid variation between samples. This leads to unwanted sources of error that can
make it problematic to interpret the results. Here we present an improved method that allows
different cell populations to be tested in the same sample. Samples are pre-labeled with
CFSE or Cy5 then mixed and stimulated to induce calcium flux. This facilitates more rapid …
currently well established flow cytometric methods, it is a tedious procedure that is difficult to
control to avoid variation between samples. This leads to unwanted sources of error that can
make it problematic to interpret the results. Here we present an improved method that allows
different cell populations to be tested in the same sample. Samples are pre-labeled with
CFSE or Cy5 then mixed and stimulated to induce calcium flux. This facilitates more rapid …