[HTML][HTML] AKT activity determines sensitivity to mammalian target of rapamycin (mTOR) inhibitors by regulating cyclin D1 and c-myc expression
JF Gera, IK Mellinghoff, Y Shi, MB Rettig, C Tran… - Journal of Biological …, 2004 - ASBMB
Prior work demonstrates that AKT activity regulates sensitivity of cells to G 1 arrest induced
by mammalian target of rapamycin (mTOR) inhibitors such as rapamycin and CCI-779. To
investigate this, a novel high-throughput microarray polysome analysis was performed to
identify genes whose mRNA translational efficiency was differentially affected following
mTOR inhibition. The analysis also allowed the assessment of steady-state transcript levels.
We identified two transcripts, cyclin D1 and c-myc, which exhibited differential expression in …
by mammalian target of rapamycin (mTOR) inhibitors such as rapamycin and CCI-779. To
investigate this, a novel high-throughput microarray polysome analysis was performed to
identify genes whose mRNA translational efficiency was differentially affected following
mTOR inhibition. The analysis also allowed the assessment of steady-state transcript levels.
We identified two transcripts, cyclin D1 and c-myc, which exhibited differential expression in …