Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells

JS Draper, K Smith, P Gokhale, HD Moore… - Nature …, 2004 - nature.com
JS Draper, K Smith, P Gokhale, HD Moore, E Maltby, J Johnson, L Meisner, TP Zwaka…
Nature biotechnology, 2004nature.com
We have observed karyotypic changes involving the gain of chromosome 17q in three
independent human embryonic stem (hES) cell lines on five independent occasions. A gain
of chromosome 12 was seen occasionally. This implies that increased dosage of
chromosome 17q and 12 gene (s) provides a selective advantage for the propagation of
undifferentiated hES cells. These observations are instructive for the future application of
hES cells in transplantation therapies in which the use of aneuploid cells could be …
Abstract
We have observed karyotypic changes involving the gain of chromosome 17q in three independent human embryonic stem (hES) cell lines on five independent occasions. A gain of chromosome 12 was seen occasionally. This implies that increased dosage of chromosome 17q and 12 gene(s) provides a selective advantage for the propagation of undifferentiated hES cells. These observations are instructive for the future application of hES cells in transplantation therapies in which the use of aneuploid cells could be detrimental.
nature.com