Purification and primary amino acid sequence of a novel neutrophil chemotactic factor LECT2
S Yamagoe, Y Yamakawa, Y Matsuo, J Minowada… - Immunology letters, 1996 - Elsevier
We purified a neutrophil chemotactic factor from a culture fluid of the PHA-activated human T-
cell leukemia SKW-3 cells. The factor showed a 16-kDa basic protein by Tricin-SDS-
polyacrylamide gel electorophoresis and analysis of amino acid composition. The primary
amino acid sequence revealed that the chemotactic factor was significantly different from
other known chemotactic factors, indicating a novel protein designated LECT2. The
sequence revealed homology with the myb-induced myeloid protein-1 (Mim-1), which is …
cell leukemia SKW-3 cells. The factor showed a 16-kDa basic protein by Tricin-SDS-
polyacrylamide gel electorophoresis and analysis of amino acid composition. The primary
amino acid sequence revealed that the chemotactic factor was significantly different from
other known chemotactic factors, indicating a novel protein designated LECT2. The
sequence revealed homology with the myb-induced myeloid protein-1 (Mim-1), which is …