The Rho/ROCK pathway mediates neurite growth-inhibitory activity associated with the chondroitin sulfate proteoglycans of the CNS glial scar
Axons fail to regenerate in the central nervous system after injury. Chondroitin sulfate
proteoglycans (CSPG) expressed in the scar significantly contribute to the nonpermissive
properties of the central nervous system environment. To examine the inhibitory activity of a
CSPG mixture on retina ganglion cell (RGC) axon growth, we employed both a stripe assay
and a nerve fiber outgrowth assay. We show that the inhibition exerted by CSPGs in vitro
can be blocked by application of either C3 transferase, a specific inhibitor of the Rho …
proteoglycans (CSPG) expressed in the scar significantly contribute to the nonpermissive
properties of the central nervous system environment. To examine the inhibitory activity of a
CSPG mixture on retina ganglion cell (RGC) axon growth, we employed both a stripe assay
and a nerve fiber outgrowth assay. We show that the inhibition exerted by CSPGs in vitro
can be blocked by application of either C3 transferase, a specific inhibitor of the Rho …