[HTML][HTML] Characterization of new cell permeable C3-like proteins that inactivate Rho and stimulate neurite outgrowth on inhibitory substrates
MJ Winton, CI Dubreuil, D Lasko, N Leclerc… - Journal of Biological …, 2002 - ASBMB
The activation state of Rho is an important determinant of axon growth and regeneration in
neurons. Axons can extend neurites on growth inhibitory substrates when Rho is inactivated
by C3-ADP-ribosyltransferase (C3). We found by Rho-GTP pull-down assay that inhibitory
substrates activate Rho. To inactivate Rho, scrape-loading of C3 was necessary because it
does not freely enter cells. To overcome the poor permeability of C3, we made and
characterized five new recombinant C3-like chimeric proteins designed to cross the cell …
neurons. Axons can extend neurites on growth inhibitory substrates when Rho is inactivated
by C3-ADP-ribosyltransferase (C3). We found by Rho-GTP pull-down assay that inhibitory
substrates activate Rho. To inactivate Rho, scrape-loading of C3 was necessary because it
does not freely enter cells. To overcome the poor permeability of C3, we made and
characterized five new recombinant C3-like chimeric proteins designed to cross the cell …