[HTML][HTML] Functional coupling between TRPC3 and RyR1 regulates the expressions of key triadic proteins
EH Lee, G Cherednichenko, IN Pessah… - Journal of biological …, 2006 - ASBMB
We have shown that TRPC3 (transient receptor potential channel canonical type 3) is
sharply up-regulated during the early part of myotube differentiation and remains elevated in
mature myotubes compared with myoblasts. To examine its functional roles in muscle,
TRPC3 was" knocked down" in mouse primary skeletal myoblasts using retroviral-delivered
small interference RNAs and single cell cloning. TRPC3 knockdown myoblasts (97.6±1.9%
reduction in mRNA) were differentiated into myotubes (TRPC3 KD) and subjected to …
sharply up-regulated during the early part of myotube differentiation and remains elevated in
mature myotubes compared with myoblasts. To examine its functional roles in muscle,
TRPC3 was" knocked down" in mouse primary skeletal myoblasts using retroviral-delivered
small interference RNAs and single cell cloning. TRPC3 knockdown myoblasts (97.6±1.9%
reduction in mRNA) were differentiated into myotubes (TRPC3 KD) and subjected to …