[HTML][HTML] Promoter-dependent effect of IKKα on NF-κB/p65 DNA binding
G Gloire, J Horion, N El Mjiyad, F Bex, A Chariot… - Journal of Biological …, 2007 - ASBMB
IKKα regulates many chromatin events in the nuclear phase of the NF-κB program, including
phosphorylation of histone H3 and removal of co-repressors from NF-κB-dependent
promoters. However, all of the nuclear functions of IKKα are not understood. In this study,
using mouse embryonic fibroblasts IKKα knock-out and reexpressing IKKα after retroviral
transduction, we demonstrate that IKKα contributes to NF-κB/p65 DNA binding activity on an
exogenous κB element and on some, but not all, endogenous NF-κB-target promoters …
phosphorylation of histone H3 and removal of co-repressors from NF-κB-dependent
promoters. However, all of the nuclear functions of IKKα are not understood. In this study,
using mouse embryonic fibroblasts IKKα knock-out and reexpressing IKKα after retroviral
transduction, we demonstrate that IKKα contributes to NF-κB/p65 DNA binding activity on an
exogenous κB element and on some, but not all, endogenous NF-κB-target promoters …