[HTML][HTML] Insulin-like growth factor I triggers nuclear accumulation of cyclin D1 in MCF-7S breast cancer cells

IHL Hamelers, RFMA van Schaik, J Sipkema… - Journal of Biological …, 2002 - ASBMB
Stimulation of the breast cancer-derived MCF-7S cell line with insulin-like growth factor I
(IGF-I; 20 ng/ml) leads to enhanced expression of cyclin D1, hyperphosphorylation of pRb,
DNA synthesis, and cell division. 17β-Estradiol (E 2; 10− 9 m) is not able to stimulate
proliferation of MCF-7S cells, although addition of E 2 to serum-starved cells does result in
induction of cyclin D1. However, in combination with submitogenic amounts of IGF-I (2
ng/ml), E 2 induces cell proliferation. We have previously shown that the synergistic action of …