[HTML][HTML] Processing and activation of pro-interleukin-16 by caspase-3
Y Zhang, DM Center, MH David, WW Cruikshank… - Journal of Biological …, 1998 - ASBMB
Interleukin-16, a proinflammatory cytokine produced in CD8+ lymphocytes, is synthesized as
a precursor protein (pro-IL-16). It is postulated that the C-terminal region of pro-IL-16 is
cleaved, releasing bioactive IL-16. To characterize IL-16 cleavage, we transfected COS cells
with a cDNA encoding a∼ 50-kDa form of pro-IL-16. Transfected COS cells released a∼ 20-
kDa IL-16 cleavage product shown to consist of the 121 C-terminal residues of pro-IL-16 by
immunoblotting and amino acid sequencing. Cleaved IL-16, but not pro-IL-16, exhibited …
a precursor protein (pro-IL-16). It is postulated that the C-terminal region of pro-IL-16 is
cleaved, releasing bioactive IL-16. To characterize IL-16 cleavage, we transfected COS cells
with a cDNA encoding a∼ 50-kDa form of pro-IL-16. Transfected COS cells released a∼ 20-
kDa IL-16 cleavage product shown to consist of the 121 C-terminal residues of pro-IL-16 by
immunoblotting and amino acid sequencing. Cleaved IL-16, but not pro-IL-16, exhibited …