Effects of exposure of human islet beta-cells to normal and high glucose levels with or without gliclazide or glibenclamide
S Del Guerra, V D'Aleo, R Lupi, M Masini… - Diabetes & …, 2009 - Elsevier
AIM: To evaluate the effects of exposure to high glucose (HG) levels and sulphonylurea on
isolated human islet-cell function, and to investigate some of the mechanisms that might be
involved. METHODS: Islet cells were isolated, using collagenase digestion and gradient
purification, from 13 pancreata from non-diabetic multiorgan donors (age: 61.2±11.5 years;
gender: 7 men/6 women; body mass index: 25.1±2.8 kg/m2). The cells were then cultured for
5 days with normal glucose (NG) concentrations (5.5 mmol/L), or NG and HG (16.7 mmol/L) …
isolated human islet-cell function, and to investigate some of the mechanisms that might be
involved. METHODS: Islet cells were isolated, using collagenase digestion and gradient
purification, from 13 pancreata from non-diabetic multiorgan donors (age: 61.2±11.5 years;
gender: 7 men/6 women; body mass index: 25.1±2.8 kg/m2). The cells were then cultured for
5 days with normal glucose (NG) concentrations (5.5 mmol/L), or NG and HG (16.7 mmol/L) …