[HTML][HTML] Identification of flow-dependent endothelial nitric-oxide synthase phosphorylation sites by mass spectrometry and regulation of phosphorylation and nitric …
B Gallis, GL Corthals, DR Goodlett, H Ueba… - Journal of Biological …, 1999 - ASBMB
Endothelial cells release nitric oxide (NO) acutely in response to increased laminar fluid
shear stress, and the increase is correlated with enhanced phosphorylation of endothelial
nitric-oxide synthase (eNOS). Phosphoamino acid analysis of eNOS from bovine aortic
endothelial cells labeled with [32 P] orthophosphate demonstrated that only phosphoserine
was present in eNOS under both static and flow conditions. Fluid shear stress induced
phosphate incorporation into two specific eNOS tryptic peptides as early as 30 s after …
shear stress, and the increase is correlated with enhanced phosphorylation of endothelial
nitric-oxide synthase (eNOS). Phosphoamino acid analysis of eNOS from bovine aortic
endothelial cells labeled with [32 P] orthophosphate demonstrated that only phosphoserine
was present in eNOS under both static and flow conditions. Fluid shear stress induced
phosphate incorporation into two specific eNOS tryptic peptides as early as 30 s after …