Analysis of protein S‐nitrosylation
CM Schonhoff, M Benhar - Current Protocols in protein science, 2011 - Wiley Online Library
Current Protocols in protein science, 2011•Wiley Online Library
S‐Nitrosylation, the redox‐based modification of cysteine thiol side chains by nitric oxide, is
a dynamic and reversible post‐translational modification of proteins that subserves many
important cellular functions. Analysis of protein S‐nitrosylation is often challenging due to
methodological limitations and the effects of various chemical and physical parameters.
Despite these technical challenges, a growing number of useful methods are now available
to analyze protein S‐nitrosylation. In this unit, several important methods to measure protein …
a dynamic and reversible post‐translational modification of proteins that subserves many
important cellular functions. Analysis of protein S‐nitrosylation is often challenging due to
methodological limitations and the effects of various chemical and physical parameters.
Despite these technical challenges, a growing number of useful methods are now available
to analyze protein S‐nitrosylation. In this unit, several important methods to measure protein …
Abstract
S‐Nitrosylation, the redox‐based modification of cysteine thiol side chains by nitric oxide, is a dynamic and reversible post‐translational modification of proteins that subserves many important cellular functions. Analysis of protein S‐nitrosylation is often challenging due to methodological limitations and the effects of various chemical and physical parameters. Despite these technical challenges, a growing number of useful methods are now available to analyze protein S‐nitrosylation. In this unit, several important methods to measure protein S‐nitrosylation and denitrosylation are discussed and evaluated. Recommendations are given regarding the potential and the applicability of the methods discussed. Curr. Protoc. Protein Sci. 63:14.6.1‐14.6.21. © 2011 by John Wiley & Sons, Inc.
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