[HTML][HTML] Kdo2-lipid A, a TLR4-specific agonist, induces de novo sphingolipid biosynthesis in RAW264. 7 macrophages, which is essential for induction of autophagy

K Sims, CA Haynes, S Kelly, JC Allegood… - Journal of biological …, 2010 - ASBMB
Activation of RAW264. 7 cells with a lipopolysaccharide specific for the TLR4 receptor, Kdo 2-
lipid A (KLA), causes a large increase in cellular sphingolipids, from 1.5 to 2.6× 10 9
molecules per cell in 24 h, based on the sum of subspecies analyzed by" lipidomic" mass
spectrometry. Thus, this study asked the following question. What is the cause of this
increase and is there a cell function connected with it? The sphingolipids arise primarily from
de novo biosynthesis based on [U-13 C] palmitate labeling, inhibition by ISP1 (myriocin) …