Influence of heme oxygenase-1 on microcirculation after kidney transplantation
JP Hölzen, C August, R Bahde, E Minin, D Lang… - Journal of Surgical …, 2008 - Elsevier
JP Hölzen, C August, R Bahde, E Minin, D Lang, S Heidenreich, KH Dietl, HU Spiegel
Journal of Surgical Research, 2008•ElsevierBACKGROUND: Cytoprotective proteins, such as heme oxygenase-1 (HO-1), play a
decisive role in ischemia-reperfusion injury during kidney transplantation. The aim of this
study was to investigate the impact of heme oxygenase-1 on microcirculation and on
ischemia-reperfusion injury in an isogenic kidney transplantation rat model. MATERIALS
AND METHODS: Seventy male Lewis rats were distributed into three groups. In Group 1
(control), the kidneys were only mobilized. In Groups 2 and 3, bilateral nephrectomy was …
decisive role in ischemia-reperfusion injury during kidney transplantation. The aim of this
study was to investigate the impact of heme oxygenase-1 on microcirculation and on
ischemia-reperfusion injury in an isogenic kidney transplantation rat model. MATERIALS
AND METHODS: Seventy male Lewis rats were distributed into three groups. In Group 1
(control), the kidneys were only mobilized. In Groups 2 and 3, bilateral nephrectomy was …
BACKGROUND
Cytoprotective proteins, such as heme oxygenase-1 (HO-1), play a decisive role in ischemia-reperfusion injury during kidney transplantation. The aim of this study was to investigate the impact of heme oxygenase-1 on microcirculation and on ischemia-reperfusion injury in an isogenic kidney transplantation rat model.
MATERIALS AND METHODS
Seventy male Lewis rats were distributed into three groups. In Group 1(control), the kidneys were only mobilized. In Groups 2 and 3, bilateral nephrectomy was performed, and a kidney from another Lewis rat was orthotopically transplanted on the left side. The donor animals in Group 3 received preconditioning with the HO-1 inductor hemin. 24 h after reperfusion graft function and morphology were examined. Microcirculation was investigated by in vivo microscopy of the renal surface 1 h after reperfusion.
RESULTS
HO-1 preconditioning led to significantly lower serum creatinine and serum urea, as well as less histological damage and inducible nitric oxide synthase expression. Microcirculation was improved by a significant enlargement of the vascular diameter and an increase of the capillary flow.
CONCLUSIONS
Treatment with hemin improves microcirculation by induction of HO-1 and reduces ischemia-reperfusion injury after kidney transplantation. HO-1 induction was shown to be a promising approach in the preconditioning of donor kidneys.
Elsevier