[PDF][PDF] Regeneration of enzyme activity by air oxidation of reduced subtilisin-modified ribonuclease

E Haber, CB Anfinsen - Journal of Biological …, 1961 - academygenbioii.pbworks.com
E Haber, CB Anfinsen
Journal of Biological Chemistry, 1961academygenbioii.pbworks.com
Regeneration of Enzyme Activity by Air Oxidation of Reduced Subtilisin-Modified Ribonuclease
Page 1 THE JOURNAL OF BIOLOGICAI, CHEMISTRY Vol. 236, No. 2, February 1961 Printed
in USA Regeneration of Enzyme Activity by Air Oxidation of Reduced Subtilisin-Modified
Ribonuclease EDGAR HABER -44~~ CHRISTIAW B. ANFINSEN From the Laboratory of
Cellular Physiology and Metabolism, National Heart Institute, National Institutes of Health,
Public Health Service, United Xtates Department of Health, Education and Welfare, Bethesda …
RESULTS
The data in Table I indicate that reduction of S-protein appears to be complete in 1 minute. At the end of this time, enzymatic activity is also completely lost. The rate of reduc-by guest, on August 29, 2010 www. jbc. org
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