A simple method for counting adherent cells: application to cultured human monocytes, macrophages and multinucleated giant cells
A Nakagawara, CF Nathan - Journal of immunological methods, 1983 - Elsevier
A simple method was devised for counting small numbers (10 4− 10 6) of adherent
mononuclear phagocytes, including populations containing multinucleated giant cells, which
often arise during cultivation of human blood monocytes. Coverslips with adherent cells
were transferred into small volumes (50–200 μl) of 0.1 M citric acid, pH 2.2, containing
0.05% naphthol blue black and 1.0% of either Triton X-100 or Cetavlon. Triton X-100 was
adequate for use with monocytes and macrophages from early cultures. However, Cetavlon …
mononuclear phagocytes, including populations containing multinucleated giant cells, which
often arise during cultivation of human blood monocytes. Coverslips with adherent cells
were transferred into small volumes (50–200 μl) of 0.1 M citric acid, pH 2.2, containing
0.05% naphthol blue black and 1.0% of either Triton X-100 or Cetavlon. Triton X-100 was
adequate for use with monocytes and macrophages from early cultures. However, Cetavlon …