Isolation and characterization of a cell line from the epithelial cells of the human fetal pancreas
S Wang, GM Beattie, MI Mally, V Cirulli, P Itkin-Ansari… - Cell Transplantation, 1997 - Elsevier
Pancreatic cell lines are useful for basic studies of pancreatic biology and for possible
application to cell transplantation therapies for diabetes. A retroviral vector expressing
simian virus 40 (SV40) T antigen and H-rasva112 was used to infect a monolayer culture of
epithelial cells from an 18-wk human fetal pancreas. Infected cells gave rise to a clonal
epithelial cell line, designated TRM-1. This cell line expresses epithelial markers as well as
glut2 and small amounts of insulin and glucagon. TRM-1 is the first cell line to be generated …
application to cell transplantation therapies for diabetes. A retroviral vector expressing
simian virus 40 (SV40) T antigen and H-rasva112 was used to infect a monolayer culture of
epithelial cells from an 18-wk human fetal pancreas. Infected cells gave rise to a clonal
epithelial cell line, designated TRM-1. This cell line expresses epithelial markers as well as
glut2 and small amounts of insulin and glucagon. TRM-1 is the first cell line to be generated …