[HTML][HTML] TAFI, or plasma procarboxypeptidase B, couples the coagulation and fibrinolytic cascades through the thrombin-thrombomodulin complex
L Bajzar, J Morser, M Nesheim - Journal of Biological Chemistry, 1996 - ASBMB
TAFI (thrombin-activatable fibrinolysis inhibitor) is a recently discovered plasma protein that
can be activated by thrombin-catalyzed proteolysis to a carboxypeptidase B-like enzyme that
inhibits fibrinolysis. This work shows that the thrombin-thrombomodulin complex, rather than
free thrombin, is the most likely physiologic activator. Thrombomodulin increases the
catalytic efficiency of the reaction by a factor of 1250, an effect expressed almost exclusively
through an increase in k cat. The kinetics of the reaction conform to a model whereby …
can be activated by thrombin-catalyzed proteolysis to a carboxypeptidase B-like enzyme that
inhibits fibrinolysis. This work shows that the thrombin-thrombomodulin complex, rather than
free thrombin, is the most likely physiologic activator. Thrombomodulin increases the
catalytic efficiency of the reaction by a factor of 1250, an effect expressed almost exclusively
through an increase in k cat. The kinetics of the reaction conform to a model whereby …