[HTML][HTML] Rac1 and Cdc42 are required for phagocytosis, but not NF-κB-dependent gene expression, in macrophages challenged with Pseudomonas aeruginosa
DJ Lee, D Cox, J Li, S Greenberg - Journal of Biological Chemistry, 2000 - ASBMB
Macrophages respond to Gram-negative bacterial pathogens by phagocytosis and pro-
inflammatory gene expression. These responses may require GTPases that have been
implicated in cytoskeletal alterations and activation of NF-κB. To determine the role of Rac1
and Cdc42 in signal transduction events triggered by Pseudomonas aeruginosa, we
expressed GTP binding-deficient alleles of Rac1 or Cdc42, or Chim-GAP, a Rac1/Cdc42-
specific GTPase-activating protein domain, in a subline of RAW 264.7 cells, and challenged …
inflammatory gene expression. These responses may require GTPases that have been
implicated in cytoskeletal alterations and activation of NF-κB. To determine the role of Rac1
and Cdc42 in signal transduction events triggered by Pseudomonas aeruginosa, we
expressed GTP binding-deficient alleles of Rac1 or Cdc42, or Chim-GAP, a Rac1/Cdc42-
specific GTPase-activating protein domain, in a subline of RAW 264.7 cells, and challenged …