HIV-1 induces phenotypic and functional perturbations of B cells in chronically infected individuals

S Moir, A Malaspina, KM Ogwaro… - Proceedings of the …, 2001 - National Acad Sciences
S Moir, A Malaspina, KM Ogwaro, ET Donoghue, CW Hallahan, LA Ehler, S Liu…
Proceedings of the National Academy of Sciences, 2001National Acad Sciences
A number of perturbations of B cells has been described in the setting of HIV infection;
however, most remain poorly understood. To directly address the effect of HIV replication on
B cell function, we investigated the capacity of B cells isolated from HIV-infected patients to
respond to a variety of stimuli before and after reduction of viremia by effective antiretroviral
therapy. B cells taken from patients with high levels of plasma viremia were defective in their
proliferative responses to various stimuli. Viremia was also associated with the appearance …
A number of perturbations of B cells has been described in the setting of HIV infection; however, most remain poorly understood. To directly address the effect of HIV replication on B cell function, we investigated the capacity of B cells isolated from HIV-infected patients to respond to a variety of stimuli before and after reduction of viremia by effective antiretroviral therapy. B cells taken from patients with high levels of plasma viremia were defective in their proliferative responses to various stimuli. Viremia was also associated with the appearance of a subpopulation of B cells that expressed reduced levels of CD21. After fractionation into CD21high- and CD21low-expressing B cells, the CD21low fraction showed dramatically reduced proliferation in response to B cell stimuli and enhanced secretion of immunoglobulins when compared with the CD21high fraction. Electron microscopic analysis of each fraction revealed cells with plasmacytoid features in the CD21low B cell population but not in the CD21high fraction. These results indicate that HIV viremia induces the appearance of a subset of B cells whose function is impaired and which may be responsible for the hypergammaglobulinemia associated with HIV disease.
National Acad Sciences