Iodo-Gen-mediated radioiodination of nucleic acids
MA Piatyszek, A Jarmolowski, J Augustyniak - Analytical biochemistry, 1988 - Elsevier
MA Piatyszek, A Jarmolowski, J Augustyniak
Analytical biochemistry, 1988•ElsevierIodo-Gen (1, 3, 4, 6-tetrachloro-3a, 6a-diphenylglycoluril), widely used as an oxidizing agent
for iodination of proteins, can also be used for iodination of nucleic acids. Optimal conditions
were determined for efficient labeling of RNA and DNA with 125I. The proposed procedure
for radioiodination of nucleic acids is more beneficial than the methods utilizing TlCl3
because of the milder reaction conditions, the simplicity and completeness of separation of
reaction products from the oxidizing agents, and the absence of a toxic catalyst. Using the …
for iodination of proteins, can also be used for iodination of nucleic acids. Optimal conditions
were determined for efficient labeling of RNA and DNA with 125I. The proposed procedure
for radioiodination of nucleic acids is more beneficial than the methods utilizing TlCl3
because of the milder reaction conditions, the simplicity and completeness of separation of
reaction products from the oxidizing agents, and the absence of a toxic catalyst. Using the …
Iodo-Gen (1,3,4,6-tetrachloro-3a,6a-diphenylglycoluril), widely used as an oxidizing agent for iodination of proteins, can also be used for iodination of nucleic acids. Optimal conditions were determined for efficient labeling of RNA and DNA with 125I. The proposed procedure for radioiodination of nucleic acids is more beneficial than the methods utilizing TlCl3 because of the milder reaction conditions, the simplicity and completeness of separation of reaction products from the oxidizing agents, and the absence of a toxic catalyst. Using the standard procedure for Iodo-Gen-mediated iodination a specific radioactivity of up to 1.3 × 109 dpm/μg RNA can be achieved. The proposed procedure is also suitable for radioiodination of DNA.
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