Rapid separation and quantitation of combined neutral and polar lipid classes by high-performance liquid chromatography and evaporative light-scattering mass …
R Homan, MK Anderson - … of Chromatography B: Biomedical Sciences and …, 1998 - Elsevier
Modifications are described for an innovative and widely used high-performance liquid
chromatography technique that resolves a very broad spectrum of lipids for quantitation by
evaporative light-scattering detection. Substitution of acetone for 2-propanol in a portion of
the solvent gradient program yields consistent resolution of diacylglycerol and cholesterol
without sacrificing baseline resolution of the remaining major lipid classes. Moreover,
previously noted instabilities in triacylglycerol retention time are eliminated. The introduction …
chromatography technique that resolves a very broad spectrum of lipids for quantitation by
evaporative light-scattering detection. Substitution of acetone for 2-propanol in a portion of
the solvent gradient program yields consistent resolution of diacylglycerol and cholesterol
without sacrificing baseline resolution of the remaining major lipid classes. Moreover,
previously noted instabilities in triacylglycerol retention time are eliminated. The introduction …