Characterization of CD8+ T cell repertoire diversity and persistence in the influenza A virus model of localized, transient infection
Seminars in immunology, 2004•Elsevier
Influenza virus infection of C57BL/6 mice provides a well-characterized model for the study
of acute CD8+ T cell responses and for the analysis of memory in the absence of antigen
persistence. The advent of tetramer reagents and intracellular cytokine staining, coupled
with techniques such as single cell RT–PCR and influenza reverse genetics, has enabled
the detailed molecular dissection of different epitope-specific primary, memory and
secondary immune CD8+ T cell responses. The approach offers novel insights into the …
of acute CD8+ T cell responses and for the analysis of memory in the absence of antigen
persistence. The advent of tetramer reagents and intracellular cytokine staining, coupled
with techniques such as single cell RT–PCR and influenza reverse genetics, has enabled
the detailed molecular dissection of different epitope-specific primary, memory and
secondary immune CD8+ T cell responses. The approach offers novel insights into the …
Influenza virus infection of C57BL/6 mice provides a well-characterized model for the study of acute CD8+ T cell responses and for the analysis of memory in the absence of antigen persistence. The advent of tetramer reagents and intracellular cytokine staining, coupled with techniques such as single cell RT–PCR and influenza reverse genetics, has enabled the detailed molecular dissection of different epitope-specific primary, memory and secondary immune CD8+ T cell responses. The approach offers novel insights into the factors determining the selection of immune repertoires, and their functional consequences for CD8+ T cell-mediated immunity.
Elsevier