Low reprogramming efficiency and reduced pluripotency have been the two major obstacles
in induced pluripotent stem (iPS) cell research. An effective and quick method to assess the
pluripotency levels of iPS cells at early stages would significantly increase the success rate
of iPS cell generation and promote its applications. We have identified a conserved
imprinted region of the mouse genome, the Dlk1-Dio3 region, which was activated in fully
pluripotent mouse stem cells but repressed in partially pluripotent cells. The degree of …